Our efforts in the area of surface engineering are directed towards controlling the interactions between synthetic materials and biological matter. To this end, we have developed a two-step strategy that starts with the modification of the surface of interest with a thin polymer coating using surface-initiated controlled radical polymerization (Step (i) in Figure 1). This coating prevents non-specific adhesion of cells and proteins to the surface, and, in a second step, can serve as a platform to introduce specific bioactive molecules (Steps (ii) – (iv) in Figure 1). Figure 1 illustrates the immobilization of AGT fusion proteins. This work is carried out in collaboration with the group of Prof. Kai Johnsson (Laboratory of Protein Engineering, Institute of Chemical Sciences and Engineering) and may lead to surfaces that could be of interest for the development of protein microarrays.
. J. Groll, J. Fiedler, E. Engelhardt, T. Ameringer, S. Tugulu, H.-A. Klok, R.E. Brenner, M. Möller, A novel PEG based surface coating for specific cell adhesion, J. Biomed. Mater. Res., 2005, in press.
. S. Tugulu, A. Arnold, I. Sielaff, K. Johnsson, H.-A. Klok, Protein functionalized polymer brushes, Biomacromolecules, 2005, in press.